Wnt-responsive Lgr5⁺ globose basal cells function as multipotent olfactory epithelium progenitor cells.
Identifieur interne : 000789 ( Main/Exploration ); précédent : 000788; suivant : 000790Wnt-responsive Lgr5⁺ globose basal cells function as multipotent olfactory epithelium progenitor cells.
Auteurs : Mengfei Chen ; Shenghe Tian ; Xiaoling Yang ; Andrew P. Lane ; Randall R. Reed [États-Unis] ; Hongjun Liu [États-Unis]Source :
- The Journal of neuroscience : the official journal of the Society for Neuroscience [ 1529-2401 ] ; 2014.
Descripteurs français
- KwdFr :
- Animaux, Animaux nouveau-nés, Antigène KI-67 (métabolisme), Capsules bactériennes (métabolisme), Cellules souches multipotentes (physiologie), Cytométrie en flux, Différenciation cellulaire (physiologie), Facteurs de transcription à motif basique hélice-boucle-hélice (métabolisme), Molécule-1 d'adhérence intercellulaire (métabolisme), Muqueuse olfactive (cytologie), Neurorécepteurs olfactifs (physiologie), Protéine GAP-43 (métabolisme), Protéine gliofibrillaire acide (métabolisme), Protéine marqueur olfactif (métabolisme), Protéines à fluorescence verte (génétique), Protéines à fluorescence verte (métabolisme), Récepteurs couplés aux protéines G (génétique), Récepteurs couplés aux protéines G (métabolisme), Souris, Souris transgéniques, beta-Galactosidase (métabolisme).
- MESH :
- cytologie : Muqueuse olfactive.
- génétique : Protéines à fluorescence verte, Récepteurs couplés aux protéines G.
- métabolisme : Antigène KI-67, Capsules bactériennes, Facteurs de transcription à motif basique hélice-boucle-hélice, Molécule-1 d'adhérence intercellulaire, Protéine GAP-43, Protéine gliofibrillaire acide, Protéine marqueur olfactif, Protéines à fluorescence verte, Récepteurs couplés aux protéines G, beta-Galactosidase.
- physiologie : Cellules souches multipotentes, Différenciation cellulaire, Neurorécepteurs olfactifs.
- Animaux, Animaux nouveau-nés, Cytométrie en flux, Souris, Souris transgéniques.
English descriptors
- KwdEn :
- Animals, Animals, Newborn, Bacterial Capsules (metabolism), Basic Helix-Loop-Helix Transcription Factors (metabolism), Cell Differentiation (physiology), Flow Cytometry, GAP-43 Protein (metabolism), Glial Fibrillary Acidic Protein (metabolism), Green Fluorescent Proteins (genetics), Green Fluorescent Proteins (metabolism), Intercellular Adhesion Molecule-1 (metabolism), Ki-67 Antigen (metabolism), Mice, Mice, Transgenic, Multipotent Stem Cells (physiology), Olfactory Marker Protein (metabolism), Olfactory Mucosa (cytology), Olfactory Receptor Neurons (physiology), Receptors, G-Protein-Coupled (genetics), Receptors, G-Protein-Coupled (metabolism), beta-Galactosidase (metabolism).
- MESH :
- chemical , genetics : Green Fluorescent Proteins, Receptors, G-Protein-Coupled.
- chemical , metabolism : Basic Helix-Loop-Helix Transcription Factors, GAP-43 Protein, Glial Fibrillary Acidic Protein, Green Fluorescent Proteins, Intercellular Adhesion Molecule-1, Ki-67 Antigen, Olfactory Marker Protein, Receptors, G-Protein-Coupled, beta-Galactosidase.
- cytology : Olfactory Mucosa.
- metabolism : Bacterial Capsules.
- physiology : Cell Differentiation, Multipotent Stem Cells, Olfactory Receptor Neurons.
- Animals, Animals, Newborn, Flow Cytometry, Mice, Mice, Transgenic.
Abstract
Persistent neurogenesis in the olfactory epithelium provides a unique model to study neural stem cell self-renewal and fate determination. In the olfactory neuroepithelium, globose basal cells (GBCs) are considered to be the direct progenitors of olfactory neurons. However, the study of neurogenesis from GBCs has been impeded by the paucity of GBC-specific markers. Here we report that Lgr5, a recently discovered adult stem cell marker, is exclusively expressed in GBCs in neonatal and adult mice. Lgr5(+) cells display characteristics of cycling stem cells, including Ki67 expression and EdU incorporation. Lineage tracing analysis demonstrates that Lgr5(+) GBCs regenerate multiple cell types under normal turnover condition or after olfactory lesion. Furthermore, upregulation or downregulation of Wnt signaling in vivo indicates a key role of Wnt signaling not only in maintaining Lgr5(+) cell proliferation and promoting neuroregeneration, but also in delaying sensory neuron maturation. Together, our observations provided new insights into the dynamics of neurogenesis in the olfactory epithelium.
DOI: 10.1523/JNEUROSCI.0240-14.2014
PubMed: 24920630
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002B09
- to stream PubMed, to step Curation: 002A93
- to stream PubMed, to step Checkpoint: 002A93
- to stream Ncbi, to step Merge: 002482
- to stream Ncbi, to step Curation: 002482
- to stream Ncbi, to step Checkpoint: 002482
- to stream Main, to step Merge: 000791
- to stream Main, to step Curation: 000789
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Wnt-responsive Lgr5⁺ globose basal cells function as multipotent olfactory epithelium progenitor cells.</title><author><name sortKey="Chen, Mengfei" sort="Chen, Mengfei" uniqKey="Chen M" first="Mengfei" last="Chen">Mengfei Chen</name><affiliation><nlm:affiliation>Department of Microbiology and Molecular Genetics and.</nlm:affiliation><wicri:noCountry code="no comma">Department of Microbiology and Molecular Genetics and.</wicri:noCountry></affiliation></author><author><name sortKey="Tian, Shenghe" sort="Tian, Shenghe" uniqKey="Tian S" first="Shenghe" last="Tian">Shenghe Tian</name><affiliation><nlm:affiliation>Department of Microbiology and Molecular Genetics and Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, and.</nlm:affiliation><wicri:noCountry code="subField">and</wicri:noCountry></affiliation></author><author><name sortKey="Yang, Xiaoling" sort="Yang, Xiaoling" uniqKey="Yang X" first="Xiaoling" last="Yang">Xiaoling Yang</name><affiliation><nlm:affiliation>Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, and.</nlm:affiliation><wicri:noCountry code="subField">and</wicri:noCountry></affiliation></author><author><name sortKey="Lane, Andrew P" sort="Lane, Andrew P" uniqKey="Lane A" first="Andrew P" last="Lane">Andrew P. Lane</name><affiliation><nlm:affiliation>Department of Otolaryngology-Head and Neck Surgery and.</nlm:affiliation><wicri:noCountry code="no comma">Department of Otolaryngology-Head and Neck Surgery and.</wicri:noCountry></affiliation></author><author><name sortKey="Reed, Randall R" sort="Reed, Randall R" uniqKey="Reed R" first="Randall R" last="Reed">Randall R. Reed</name><affiliation wicri:level="2"><nlm:affiliation>Center for Sensory Biology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Center for Sensory Biology, Johns Hopkins University School of Medicine, Baltimore</wicri:cityArea></affiliation></author><author><name sortKey="Liu, Hongjun" sort="Liu, Hongjun" uniqKey="Liu H" first="Hongjun" last="Liu">Hongjun Liu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Molecular Genetics and Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, and liuh4@pitt.edu.</nlm:affiliation><country wicri:rule="url">États-Unis</country><wicri:regionArea>Department of Microbiology and Molecular Genetics and Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213</wicri:regionArea><wicri:noRegion>Pennsylvania 15213</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="RBID">pubmed:24920630</idno><idno type="pmid">24920630</idno><idno type="doi">10.1523/JNEUROSCI.0240-14.2014</idno><idno type="wicri:Area/PubMed/Corpus">002B09</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002B09</idno><idno type="wicri:Area/PubMed/Curation">002A93</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002A93</idno><idno type="wicri:Area/PubMed/Checkpoint">002A93</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002A93</idno><idno type="wicri:Area/Ncbi/Merge">002482</idno><idno type="wicri:Area/Ncbi/Curation">002482</idno><idno type="wicri:Area/Ncbi/Checkpoint">002482</idno><idno type="wicri:Area/Main/Merge">000791</idno><idno type="wicri:Area/Main/Curation">000789</idno><idno type="wicri:Area/Main/Exploration">000789</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Wnt-responsive Lgr5⁺ globose basal cells function as multipotent olfactory epithelium progenitor cells.</title><author><name sortKey="Chen, Mengfei" sort="Chen, Mengfei" uniqKey="Chen M" first="Mengfei" last="Chen">Mengfei Chen</name><affiliation><nlm:affiliation>Department of Microbiology and Molecular Genetics and.</nlm:affiliation><wicri:noCountry code="no comma">Department of Microbiology and Molecular Genetics and.</wicri:noCountry></affiliation></author><author><name sortKey="Tian, Shenghe" sort="Tian, Shenghe" uniqKey="Tian S" first="Shenghe" last="Tian">Shenghe Tian</name><affiliation><nlm:affiliation>Department of Microbiology and Molecular Genetics and Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, and.</nlm:affiliation><wicri:noCountry code="subField">and</wicri:noCountry></affiliation></author><author><name sortKey="Yang, Xiaoling" sort="Yang, Xiaoling" uniqKey="Yang X" first="Xiaoling" last="Yang">Xiaoling Yang</name><affiliation><nlm:affiliation>Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, and.</nlm:affiliation><wicri:noCountry code="subField">and</wicri:noCountry></affiliation></author><author><name sortKey="Lane, Andrew P" sort="Lane, Andrew P" uniqKey="Lane A" first="Andrew P" last="Lane">Andrew P. Lane</name><affiliation><nlm:affiliation>Department of Otolaryngology-Head and Neck Surgery and.</nlm:affiliation><wicri:noCountry code="no comma">Department of Otolaryngology-Head and Neck Surgery and.</wicri:noCountry></affiliation></author><author><name sortKey="Reed, Randall R" sort="Reed, Randall R" uniqKey="Reed R" first="Randall R" last="Reed">Randall R. Reed</name><affiliation wicri:level="2"><nlm:affiliation>Center for Sensory Biology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Center for Sensory Biology, Johns Hopkins University School of Medicine, Baltimore</wicri:cityArea></affiliation></author><author><name sortKey="Liu, Hongjun" sort="Liu, Hongjun" uniqKey="Liu H" first="Hongjun" last="Liu">Hongjun Liu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Molecular Genetics and Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, and liuh4@pitt.edu.</nlm:affiliation><country wicri:rule="url">États-Unis</country><wicri:regionArea>Department of Microbiology and Molecular Genetics and Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213</wicri:regionArea><wicri:noRegion>Pennsylvania 15213</wicri:noRegion></affiliation></author></analytic><series><title level="j">The Journal of neuroscience : the official journal of the Society for Neuroscience</title><idno type="eISSN">1529-2401</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Animals, Newborn</term><term>Bacterial Capsules (metabolism)</term><term>Basic Helix-Loop-Helix Transcription Factors (metabolism)</term><term>Cell Differentiation (physiology)</term><term>Flow Cytometry</term><term>GAP-43 Protein (metabolism)</term><term>Glial Fibrillary Acidic Protein (metabolism)</term><term>Green Fluorescent Proteins (genetics)</term><term>Green Fluorescent Proteins (metabolism)</term><term>Intercellular Adhesion Molecule-1 (metabolism)</term><term>Ki-67 Antigen (metabolism)</term><term>Mice</term><term>Mice, Transgenic</term><term>Multipotent Stem Cells (physiology)</term><term>Olfactory Marker Protein (metabolism)</term><term>Olfactory Mucosa (cytology)</term><term>Olfactory Receptor Neurons (physiology)</term><term>Receptors, G-Protein-Coupled (genetics)</term><term>Receptors, G-Protein-Coupled (metabolism)</term><term>beta-Galactosidase (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Animaux nouveau-nés</term><term>Antigène KI-67 (métabolisme)</term><term>Capsules bactériennes (métabolisme)</term><term>Cellules souches multipotentes (physiologie)</term><term>Cytométrie en flux</term><term>Différenciation cellulaire (physiologie)</term><term>Facteurs de transcription à motif basique hélice-boucle-hélice (métabolisme)</term><term>Molécule-1 d'adhérence intercellulaire (métabolisme)</term><term>Muqueuse olfactive (cytologie)</term><term>Neurorécepteurs olfactifs (physiologie)</term><term>Protéine GAP-43 (métabolisme)</term><term>Protéine gliofibrillaire acide (métabolisme)</term><term>Protéine marqueur olfactif (métabolisme)</term><term>Protéines à fluorescence verte (génétique)</term><term>Protéines à fluorescence verte (métabolisme)</term><term>Récepteurs couplés aux protéines G (génétique)</term><term>Récepteurs couplés aux protéines G (métabolisme)</term><term>Souris</term><term>Souris transgéniques</term><term>beta-Galactosidase (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Green Fluorescent Proteins</term><term>Receptors, G-Protein-Coupled</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Basic Helix-Loop-Helix Transcription Factors</term><term>GAP-43 Protein</term><term>Glial Fibrillary Acidic Protein</term><term>Green Fluorescent Proteins</term><term>Intercellular Adhesion Molecule-1</term><term>Ki-67 Antigen</term><term>Olfactory Marker Protein</term><term>Receptors, G-Protein-Coupled</term><term>beta-Galactosidase</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Muqueuse olfactive</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Olfactory Mucosa</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines à fluorescence verte</term><term>Récepteurs couplés aux protéines G</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Bacterial Capsules</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Antigène KI-67</term><term>Capsules bactériennes</term><term>Facteurs de transcription à motif basique hélice-boucle-hélice</term><term>Molécule-1 d'adhérence intercellulaire</term><term>Protéine GAP-43</term><term>Protéine gliofibrillaire acide</term><term>Protéine marqueur olfactif</term><term>Protéines à fluorescence verte</term><term>Récepteurs couplés aux protéines G</term><term>beta-Galactosidase</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Cellules souches multipotentes</term><term>Différenciation cellulaire</term><term>Neurorécepteurs olfactifs</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Cell Differentiation</term><term>Multipotent Stem Cells</term><term>Olfactory Receptor Neurons</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Animals, Newborn</term><term>Flow Cytometry</term><term>Mice</term><term>Mice, Transgenic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Animaux nouveau-nés</term><term>Cytométrie en flux</term><term>Souris</term><term>Souris transgéniques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Persistent neurogenesis in the olfactory epithelium provides a unique model to study neural stem cell self-renewal and fate determination. In the olfactory neuroepithelium, globose basal cells (GBCs) are considered to be the direct progenitors of olfactory neurons. However, the study of neurogenesis from GBCs has been impeded by the paucity of GBC-specific markers. Here we report that Lgr5, a recently discovered adult stem cell marker, is exclusively expressed in GBCs in neonatal and adult mice. Lgr5(+) cells display characteristics of cycling stem cells, including Ki67 expression and EdU incorporation. Lineage tracing analysis demonstrates that Lgr5(+) GBCs regenerate multiple cell types under normal turnover condition or after olfactory lesion. Furthermore, upregulation or downregulation of Wnt signaling in vivo indicates a key role of Wnt signaling not only in maintaining Lgr5(+) cell proliferation and promoting neuroregeneration, but also in delaying sensory neuron maturation. Together, our observations provided new insights into the dynamics of neurogenesis in the olfactory epithelium.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Maryland</li></region></list><tree><noCountry><name sortKey="Chen, Mengfei" sort="Chen, Mengfei" uniqKey="Chen M" first="Mengfei" last="Chen">Mengfei Chen</name><name sortKey="Lane, Andrew P" sort="Lane, Andrew P" uniqKey="Lane A" first="Andrew P" last="Lane">Andrew P. Lane</name><name sortKey="Tian, Shenghe" sort="Tian, Shenghe" uniqKey="Tian S" first="Shenghe" last="Tian">Shenghe Tian</name><name sortKey="Yang, Xiaoling" sort="Yang, Xiaoling" uniqKey="Yang X" first="Xiaoling" last="Yang">Xiaoling Yang</name></noCountry><country name="États-Unis"><region name="Maryland"><name sortKey="Reed, Randall R" sort="Reed, Randall R" uniqKey="Reed R" first="Randall R" last="Reed">Randall R. Reed</name></region><name sortKey="Liu, Hongjun" sort="Liu, Hongjun" uniqKey="Liu H" first="Hongjun" last="Liu">Hongjun Liu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Amérique/explor/PittsburghV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000789 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000789 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Amérique
|area= PittsburghV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:24920630
|texte= Wnt-responsive Lgr5⁺ globose basal cells function as multipotent olfactory epithelium progenitor cells.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:24920630" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PittsburghV1
| This area was generated with Dilib version V0.6.38. |  |